Unless otherwise specified, all products are for Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale.
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US Orders: 877.232.8995
Table 2. Essential control assay tubes
Tube No.
1 (Negative Control
0 pg/ml Standards)
2 (20 pg/ml Standards)
3 (40 pg/ml Standards)
4 (80 pg/ml Standards)
5 (156 pg/ml Standards)
6 (312 pg/ml Standards)
7 (625 pg/ml Standards)
8 (1250 pg/ml Standards)
9 (2500 pg/ml Standards)
10 (5000 pg/ml Standards)
mixed Capture Beads, Assay Diluent, PE Detection Reagent
mixed Capture Beads, Inflammation Standards 1:256 Dilution, PE Detection Reagent
mixed Capture Beads, Inflammation Standards 1:128 Dilution, PE Detection Reagent
mixed Capture Beads, Inflammation Standards 1:64 Dilution, PE Detection Reagent
mixed Capture Beads, Inflammation Standards 1:32 Dilution, PE Detection Reagent
mixed Capture Beads, Inflammation Standards 1:16 Dilution, PE Detection Reagent
mixed Capture Beads, Inflammation Standards 1:8 Dilution, PE Detection Reagent
mixed Capture Beads, Inflammation Standards 1:4 Dilution, PE Detection Reagent
mixed Capture Beads, Inflammation Standards 1:2 Dilution, PE Detection Reagent
mixed Capture Beads, Inflammation Standards "Top Standard", PE Detection Reagent
Reagents
(All reagent volumes are 50 µl)
Cytometer Setup, Data Acquisition, and Analysis
The Cytometer setup information in this section is for the BD FACScan™ and
BD FACSCalibur™ flow cytometers. The BD FACSComp™ software is useful for
setting up the flow cytometer. BD CellQuest™ software is required for analyzing
samples and formatting data for subsequent analysis using the BD™ CBA Software.
Preparation of Cytometer Setup Beads
1. Add 50 µl of Cytometer Setup Beads to three cytometer setup tubes labeled
A, B, and C.
2. Add 50 µl of FITC Positive Control Detector to tube B.
3. Add 50 µl of PE Positive Control Detector to tube C.
4. Incubate tubes A, B, and C for 30 minutes at room temperature and
protect from direct exposure to light.
5. Add 450 µl of Wash Buffer to tube A and 400 µl of Wash Buffer to
tubes B and C.
6. Proceed to Instrument Setup with BD FACSComp™ software and
BD CaliBRITE™ beads.
