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Linearity
In two experiments, the following matrices were spiked with IL-6, IL-10, MCP-1,
IFN-γ, TNF, and IL-12p70 and were then serially diluted with Assay Diluent.
Dilution
1:4
1:8
1:16
1:32
1:64
1:128
1:256
1:512
1:1024
Slope
Neat
1:2
1:4
1:8
1:16
1:32
1:64
1:128
1:256
Slope
Dilution
1:4
1:8
1:16
1:32
1:64
1:128
1:256
1:512
1:1024
Slope
Neat
1:2
1:4
1:8
1:16
1:32
1:64
1:128
1:256
Slope
Observed
IL-6 (pg/ml)
4041.8
2128.9
1086.9
529.3
277.1
136.9
70.6
33.2
14.5
1.006
4347.7
2370.3
1207.6
572.5
295.0
132.3
68.9
39.8
17.5
0.998
Observed
IFN-γ (pg/ml)
2877.2
1588.3
958.8
520.4
255.4
140.1
69.9
34.2
18.1
0.922
4518.4
2392.4
1255.9
640.7
303.3
145.3
72.2
38.6
20.9
0.999
Matrix
Pooled mouse sera
1:4 starting dilution
Cell culture medium
Matrix
Pooled mouse sera
1:4 starting dilution
Cell culture medium
Observed
IL-10 (pg/ml)
1935.9
1244.7
797.3
438.6
238.7
136.7
76.7
29.9
14.2
0.882
4870.6
2373.0
1271.9
588.9
312.8
151.0
72.9
44.2
21.6
0.979
Observed
TNF (pg/ml)
1601.9
1001.2
630.0
370.7
211.5
122.0
73.4
38.0
17.5
0.801
4407.9
2576.8
1330.1
616.1
321.2
146.3
75.8
43.2
21.6
0.979
Observed
MCP-1 (pg/ml)
4052.3
2227.9
1183.0
619.2
291.8
160.5
77.8
22.3
0.0
1.029
4946.1
2379.3
1202.2
593.1
296.4
135.7
57.8
34.6
18.1
1.026
Observed
IL-12p70 (pg/ml)
3372.1
1675.3
913.5
491.7
242.8
142.2
82.7
34.4
12.9
0.961
4615.7
2426.8
1203.6
588.7
328.2
130.6
63.0
44.5
19.1
1.113