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Not for use in diagnostic or therapeutic procedures. Not for resale.
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Precision
Intra-assay: Ten replicates of each of three different levels of IL-6, IL-10, MCP-1,
IFN-γ, TNF, and IL-12p70 were tested.
Protein
Actual Mean
Conc. (pg/ml):
SD
% CV
IL-6 IL-10
Protein
Actual Mean
Conc. (pg/ml):
SD
% CV
MCP-1 IFN-γ
Protein
Actual Mean
Conc. (pg/ml):
SD
% CV
TNF IL-12p70
76 551 2107 78 573 1987
4 20 94 7 19 104
5% 4% 5% 9% 3% 5%
66 614 2300 73 633 2239
6 9 46 3 19 103
10% 2% 2% 4% 3% 5%
69 597 2358 66 600 2238
2 21 103 10 23 103
3% 4% 4% 15% 4% 5%
Inter-assay: Three different levels of IL-6, IL-10, MCP-1, IFN-γ, TNF, and
IL-12p70 (80, 625 and 2500 pg/ml) were tested in four experiments.
Protein
Number of
Replicates:
Actual Mean
Conc. (pg/ml):
SD
% CV
IL-6 IL-10
8 8 8 8 8 8
79 557 2426 71 581 2329
8 62 198 10 45 162
10% 11% 8% 14% 8% 7%
Protein
Number of
Replicates:
Actual Mean
Conc. (pg/ml):
SD
% CV
MCP-1 IFN-γ
8 8 8 8 8 8
58 598 2431 75 631 2425
11 33 51 6 40 120
19% 5% 2% 8% 6% 5%
Protein
Number of
Replicates:
Actual Mean
Conc. (pg/ml):
SD
% CV
TNF IL-12
8 8 8 8 8 8
75 592 2697 72 590 2430
8 40 155 7 30 70
11% 7% 6% 9% 5% 3%
Note: The number of replicates refers to the total number of assay
tubes tested at a given concentration of protein.