Unless otherwise specified, all products are for Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale.
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US Orders: 877.232.8995
Reagents Provided
Bead Reagents
Mouse Inflammation Capture Beads (A1 – A6): The specific capture beads, having
discrete fluorescence intensity characteristics, are distributed from brightest to
dimmest as follows:
Bead
(Brightest) A1
A2
A3
A4
A5
(Dimmest) A6
Specificity
IL-6
IL-10
MCP-1
IFN-γ
TNF
IL-12p70
A single 80-test vial of each specific capture bead (A1 – A6) is included in this kit.
Store at 4°C. Do not freeze.
Note: The antibody-conjugated beads will settle out of suspension over
time. It is necessary to vortex the vial vigorously for 3 – 5 seconds
before taking a bead suspension aliquot.
Cytometer Setup Beads (D): A single, 30-test vial of setup beads for setting the
initial instrument PMT voltages and compensation settings is sufficient for 10
instrument setup procedures. The Cytometer Setup Beads are formulated for
use at 50 µl/test.
Antibody and Standard Reagents
Mouse Inflammation PE Detection Reagent (B): An 80-test vial of PE-conjugated
anti-mouse IL-6, IL-10, MCP-1, IFN-γ, TNF, and IL-12p70 antibodies that is
formulated for use at 50 µl/test. Store at 4°C. Do not freeze.
Mouse Inflammation Standards (C): Two vials containing lyophilized recombinant
mouse proteins. Each vial should be reconstituted in 2.0 ml of Assay Diluent to
prepare the top standard. Store at 4°C.
PE Positive Control Detector (E1): A 10-test vial of PE-conjugated antibody control
that is formulated for use at 50 µl/test. This reagent is used with the Cytometer
Setup Beads to set the initial instrument compensation settings. Store at 4°C.
Do not freeze.
FITC Positive Control Detector (E2): A 10-test vial of FITC-conjugated antibody
control that is formulated for use at 50 µl/test. This reagent is used with the
Cytometer Setup Beads to set the initial instrument compensation settings.
Store at 4°C. Do not freeze.
