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Adjust gate R5 as necessary so that the FL1 bright bead population is located in
gate R5 (Figure 3d). Using the FL2 – %FL1 control, adjust the median of R5 to
equal the median of R4 (Figure 3d).
(R4) Median: 2.94 (R5) Median: 3.75
Figure 3d
Run Cytometer Setup Beads tube C to adjust the compensation settings for
FL1 – %FL2 and FL3 – %FL2.
Adjust gate R7 so that the FL2 bright bead population is located in gate R7
(Figure 3e). Using the FL1 – %FL2 control, adjust the median of R7 to equal
the median of R6 (Figure 3e).
(R6) Median: 2.81 (R7) Median: 3.08
Figure 3e
Adjust gate R9 so that the FL2 bright bead population is located in gate R9
(Figure 3f). Using the FL3 – %FL2 control, adjust the median of R9 to equal
the median of R8 (Figure 3f).
(R8) Median: 27.88 (R9) Median: 28.13
Figure 3f
Set the FL2 – %FL3 to 0.1 if necessary. Save and print the optimized instrument
settings.
