Unless otherwise specified, all products are for Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale.
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Performance
The BD™ CBA Mouse Inflammation kit has been rigorously tested for the
following: limit of detection, spike recovery, dilution linearity, specificity, and
intra- and inter-assay precision.
Recovery and linearity results for cell-culture supernatant are using the culture
supernatant assay procedure, and the serum/plasma recovery and linearity results
are using the serum/plasma assay protocol.
Limit of Detection
The individual standard curve range for a given protein defines the minimum
and maximum quantifiable levels using the BD CBA Mouse Inflammation kit (ie,
20 pg/ml and 5000 pg/ml). By applying the 4-parameter curve fit option, it
is possible to extrapolate values for sample intensities not falling within
the limits of the standard curve. It is up to the researcher to decide the best
method for calculating values for unknown samples using this assay. The limit
of detection for each protein using the BD CBA Mouse Inflammation kit is
defined as the corresponding concentration at two standard deviations above the
median fluorescence of 20 replicates of the negative control (0 pg/ml).
Protein
IL-6
IL-10
MCP-1
IFN-γ
TNF
IL-12p70
Median Fluorescence
3.3
5.9
9.3
5.4
6.5
4.1
Standard Deviation
0.3
0.5
0.4
0.4
0.3
0.4
Limit of Detection
(pg/ml)
5
17.5
52.7
2.5
7.3
10.7
