Unless otherwise specified, all products are for Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale.
19
US Orders: 877.232.8995
Analysis of Sample Data
The analysis of BD™ CBA data is optimized when using the BD CBA Software.
Install the software according to the instructions in the Software User’s Guide.
1. Transfer the FACS file data for the experiment to the computer with the
BD CBA Software.
2. Create two new file folders and label one “Standards” and the other “Samples”.
3. Move data files to the appropriate folders.
Note: Only the files for control assay tubes no. 1 – 10 (the PE Detection
Reagent alone and the dilution of standards) should be moved to
the “Standards” file folder. All other samples should be moved to
the “Samples” file folder.
Follow the instructions for analysis given in the BD™ CBA Software User’s Guide.
Note: When entering analyte concentrations for the standards used in
the experiment, it is necessary to give names to each analyte.
For the BD CBA Mouse Inflammation, analyte 1 is IL-12p70,
analyte 2 is TNF, analyte 3 is IFN-γ, analyte 4 is MCP-1,
analyte 5 is IL-10, and analyte 6 is IL-6.
Typical Data
Negative Control
A.
Standards 80 pg/ml
B.
Standards 625 pg/ml
C.
Standards 5000 pg/ml
D.
Figure 5. BD CellQuest
™
Data Examples for Standards and Detectors Alone
