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Not for use in diagnostic or therapeutic procedures. Not for resale.
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Introduction
Flow cytometry is an analysis tool that allows for the discrimination of different
particles on the basis of size and color. Multiplexing is the simultaneous assay of
many analytes in a single sample. The BD™ Cytometric Bead Array (CBA) employs
a series of particles with discrete fluorescence intensities to simultaneously detect
multiple soluble analytes. The BD™ CBA is combined with flow cytometry to
create a powerful multiplexed assay.
The BD CBA system uses the sensitivity of amplified fluorescence detection by
flow cytometry to measure soluble analytes in a particle-based immunoassay.
Each bead in a BD CBA provides a capture surface for a specific protein and is
analogous to an individually coated well in an ELISA plate. The BD CBA capture
bead mixture is in suspension to allow for the detection of multiple analytes in
a small volume sample. The combined advantages of the broad dynamic range
of fluorescence detection via flow cytometry and the efficient capturing of
analytes via suspended particles enable BD CBA to use fewer sample dilutions
and to obtain the value of an unknown in substantially less time (compared to
conventional ELISA).
The BD™ CBA Mouse Inflammation Kit can be used to quantitatively measure
Interleukin-6 (IL-6), Interleukin-10 (IL-10), Monocyte Chemoattractant Protein-1
(MCP-1), Interferon-γ (IFN-γ), Tumor Necrosis Factor (TNF), and Interleukin-12p70
(IL-12p70) protein levels in a single sample. The kit performance has been optimized
for analysis of specific proteins in tissue culture supernatants, EDTA plasma, and
serum samples.
