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1. Reconstitute Mouse Inflammation Standards in
Assay Diluent (15 min)
2. Dilute Standards by serial dilutions using the Assay Diluent
2 Hour incubation at RT
30 minute
incubation at RT
(protect from light)
3. Mix 10 µl/test of each Mouse Inflammation Capture
Bead suspension (vortex before aliquoting)
4. Transfer 50 µl of mixed beads to each assay tube
6. Add PE Detection Reagent (50 µl/test)
5. Add Standard Dilutions and test samples to the
appropriate sample tubes (50 µl/tube)
7. Wash samples with 1 ml Wash Buffer and centrifuge
8. Add 300 µl of Wash Buffer to each assay tubes
and analyze samples
(protect from light)
3. Add 400 µl of Wash Buffer to
tubes B and C
4. Add 450 µl of Wash Buffer to
tube A
5. Use tubes A, B and C for
cytometer setup
Cytometer Setup Bead Procedure
1. Add Cytometer Setup Beads
(vortex before adding) to setup
tubes A, B and C (50 µl/tube)
2. Add 50 µl of FITC Positive
Control to tube B and 50 µl of
PE Positive Control to tube C
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