Unless otherwise specified, all products are for Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale.
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Buffer Reagents
Wash Buffer (F): A single, 130 ml bottle of phosphate buffered saline (PBS)
solution (1×), containing protein* and detergent, used for wash steps and to
resuspend the washed beads for analysis. Store at 4°C.
Assay Diluent (G): A single, 30 ml bottle of a buffered protein* solution (1×) used
to reconstitute and dilute the Mouse Inflammation Standards and to dilute test
samples. Store at 4°C.
Warnings and Precautions
Hazardous Ingredients:
Sodium Azide:
Components A1 - A6, B, D, E1 – E2, F, and G contain 0.09%
sodium azide. Sodium azide yields a highly toxic hydrazoic acid
under acidic conditions. Dilute azide compounds in running
water before discharging to avoid accumulation of potentially
explosive deposits in plumbing.
* Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Materials Required but not Provided
In addition to the reagents provided in the BD™ CBA Mouse Inflammation Kit,
the following items are also required:
• A flow cytometer equipped with a 488-nm laser capable of detecting and
distinguishing fluorescence emissions at 576 and 670 nm (eg, BD FACScan™
or BD FACSCalibur™ instruments) and BD CellQuest™ software.
• 12
× 75 mm sample acquisition tubes for a flow cytometer
(eg, BD Falcon™ Cat. No. 352008.)
• BD CBA Software, (Cat. No. 550065) or FCAP Array™ software
(Cat. No. 641488).
Note: Microsoft® Excel and a Macintosh or PC-compatible computer
are required to utilize the BD CBA Software. See the BD CBA
Software User’s Guide for details.
• BD CaliBRITE™ 3 Beads, (Cat. No. 340486).
