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Recovery
Individual protein was spiked into various matrices at three different levels within
the assay range. The cell culture media used in these experiments were not diluted
before addition of the protein. The pooled mouse serum samples in these experiments
were diluted 1:4 in Assay Diluent before addition of the cytokine protein. Results
are compared with the same concentrations of the proteins spiked in the Assay
Diluent, as follows:
Matrix
Pooled mouse sera
1:4 dilution
Standard Spike
Concentration (pg/ml)
Protein
IL-6
IL-6
IL-10
MCP-1
MCP-1
IFN-γ
IFN-γ
TNF
TNF
IL-12p70
IL-12p70
Observed in Given
Matrix (pg/ml)
% Recovery
Cell culture media
IL-10
Pooled mouse sera
1:4 dilution
Cell culture media
Pooled mouse sera
1:4 dilution
Cell culture media
Pooled mouse sera
1:4 dilution
Cell culture media
Pooled mouse sera
1:4 dilution
Cell culture media
Pooled mouse sera
1:4 dilution
Cell culture media
2500
625
80
2500
625
80
2500
625
80
2500
625
80
2500
625
80
2500
625
80
2500
625
80
2500
625
80
2500
625
80
2500
625
80
2500
625
80
2500
625
80
1990.3
458.4
62.3
2476.7
594.3
95.8
918.5
208.2
11.2
2326.7
581.9
99.7
1954.5
448.5
35.8
2474.2
579.2
82.7
1386.8
323.9
46.6
2423.9
584.8
79.7
779.8
181.0
26.8
2651.7
587.5
83.3
1468.5
350.5
23.5
2349.0
538.2
73.9
80%
73%
78%
99%
95%
120%
37%
33%
14%
93%
93%
125%
78%
72%
45%
99%
93%
103%
55%
52%
58%
97%
94%
100%
31%
29%
34%
106%
94%
104%
59%
56%
29%
94%
86%
92%