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Microplate Luminometer Maintenance
98
8.6 Priming/Washing/Cleaning the Injector Tubings
Washing and priming of the injector tubings are controlled via
the Simplicity PC software.
To clean the injector tubing system you should rinse it regularly
with distilled or deionized water. Further recommendations for
cleaning see section 8.8.
For measurement injections you must first prime the tubing, so
that the full volume is injected with the first shot.
Proceed as follows to wash or prime the tubing systems:
1. Initialize the injector unit
Select any measurement protocol that contains a measure-
ment programmed with the respective injectors; click <Run>
in the Protocol Manager.
Click the Initialize button in the measurement menu. (
)
This opens a window prompting you to place the plastic tub in
the microplate loading compartment (instead of a microplate)
to collect any escaping liquid.
As soon as you have positioned the plastic tub, click <OK>.
The end of the initialization is indicated in another window.
2. Washing and Priming the Injector Tubings
Make sure there is still enough space in the plastic tub for the
escaping wash fluid.
Click the Wash button to open the Parameters for Priming
Injectors dialog box. (
)
Select the injector (Inj. 1 or Inj. 2).
Enter the volume/stroke (ml) and the number of strokes for
washing and priming.
The programmed procedure starts as soon as you click
<OK>.
You may stop a started wash cycle any time by clicking the Stop
Priming button. (
)