Microplate Luminometer Maintenance
101
8.9 Promega Reagents Cleaning Procedure
One of the Promega Dual Luciferase Assay reagents, the Stop &
Glo Reagent, has a slight affinity to plastic materials. To avoid
cross contamination when changing the injector tubings, we
recommend the follow the cleaning procedure outlined below.
To keep the fluids in the syringes for about 30 minutes, move
the syringe pistons to the extended position and hold them
there. To do this you must use a cleaning protocol.
Therefore, create your own cleaning protocol from the protocol
you are using for measurement: set the injector volumes as
150 µl and then save the protocol under another name (e.g.
cleaning_DLR_Assay).
Proceed as follows:
1. Wash with deionized or distilled water
1.1 Use deionized or distilled water to remove the reagents
remaining in the injection system.
1.2 Place the priming container on the microplate loading area.
1.3 Start the cleaning protocol and initialize the injectors.
1.4 Click the <Priming> button and select the injector(s) you
want to clean.
1.5 Select a volume of 150 µl and 20 injection cycles (strokes).
1.6 Click <OK> to start the wash process.
1.7 Repeat steps 1.1 - 1.6.
2. Cleaning with Ethanol
2.1 Fill the reagent bottles and injector(s) with 70% Ethanol.
2.2 Remove front cover of injector unit to watch the syringe
movement.
2.3 Click the <Priming> button and select the injector(s) you
want to clean.
2.4 Select a volume of 150 µl and 20 injection cycles (strokes).
2.5 Click <OK> to start the wash process.
2.6 Repeat steps 2.3 – 2.5.
3. Re-washing with deionized or distilled water.
3.1 Wash the syringe with deionized or distilled water, using
the priming routine (steps 1.1 – 1.7) (20 cycles (strokes)
with 150 µl each).
3.2 Repeat the wash process with deionized or distilled water
to remove all traces of ethanol.
