Microplate Luminometer System Description
11
In contrast to other physical units, luminescence is not indicated
in fixed units of measure, for example, Lumen, but in "Relative
Light Units" (RLU). Therefore, only results from the same lumi-
nometer type can be compared with each other. An RLU factor
has been defined in order to compare results from different lu-
minometers. This factor multiplies all results. Typically, the RLU
factor is 1. Upon request, this RLU factor can be changed.
2.2.5 Background Measurement and Subtraction
The background is comprised of two components: instrument
background and reagent background.
The reagent background is obtained by measuring reagents
without any analyte. For example, with a reporter gene assays,
this measurement is of the reagents measured without cell
lysate.
2.2.6 Injector Compartment behind the Service Door
When your system includes injectors, the injector compartment
of the MONOLIGHT 3096 located behind the service door next to
the instrument door is equipped as follows:
The service door is located to the right next to the instrument
door. On the right-hand side it is held by Velcro fastening. The
plate covers the instrument area with the injector tubings, the
injector tip holder and the measurement head (see Figure 2-5).
Caution: Since the photomultiplier is exposed to incident light
when the service door is opened, you must disconnect the in-
strument from main before opening it to rule out damage to the
photomultiplier. For measurement the service door and the in-
strument door must be closed properly! Never operate the in-
strument with the service door removed.
• Before opening the locking plate next to the instrument door,
disconnect the instrument from wall socket and make sure
the instrument door is open!
• First, open the 2 fastening screws of the locking plate and
hold the plate at the screwed side and take it off! Otherwise,
the locking plate may get damaged!
RLU
